Coding

Part:BBa_K3602015:Design

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


Cas12a w. tags, optimized for human cells


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 349
    Illegal PstI site found at 1411
    Illegal PstI site found at 3820
    Illegal PstI site found at 4018
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 349
    Illegal PstI site found at 1411
    Illegal PstI site found at 3820
    Illegal PstI site found at 4018
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 533
    Illegal BglII site found at 2075
    Illegal BglII site found at 2384
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 349
    Illegal PstI site found at 1411
    Illegal PstI site found at 3820
    Illegal PstI site found at 4018
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 349
    Illegal PstI site found at 1411
    Illegal PstI site found at 3820
    Illegal PstI site found at 4018
    Illegal NgoMIV site found at 3619
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 131


Design Notes

This sequence is designed with the 6x His Tag/Twin Strep/SUMO tags for purification.


Source

This sequence is from the PhyLife group of the University of Southern Denmark, where it was originally used for human cells.

References